| 名称 | 组蛋白H2B(H2B)酶联免疫吸附检测试剂盒 |
| 检测物种 | Human,Rat,Mouse |
| 别称 | HIST1H2BA; testis; bA317E16.3; H2B; H2B histone family member U; H2B histone family member U testis specific; H2B histone family, member U, (testis specific); H2B testis; H2B1A_HUMAN; H2BFU; H2BT; HIST1H2BA; Histone 1, H2ba; Histone cluster 1 H2ba; Histone H2B; Histone H2B testis; Histone H2B type 1 A; Histone H2B type 1-A; Histone H2B type 1A; STBP; Testis specific histone H2B; Testis-specific histone H2B; TSH 2B; TSH2B; TSH2B.1; |
| 反应类型 | Sandwich |
| 灵敏度 | 1.15 ng/mL |
| 检测范围 | 3.13-200 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | 本试剂盒采用夹心法原理。将特异性抗人,小鼠,大鼠 H2B抗体包被于96孔微孔板中,向微孔中分别加入人,小鼠,大鼠 H2B标准品或样本,使标准品中的人,小鼠,大鼠 H2B蛋白或样本中的人,小鼠,大鼠 H2B蛋白与固相于微孔板上的抗人,小鼠,大鼠 H2B抗体结合,然后加入生物素化抗人,小鼠,大鼠 H2B抗体,将未结合的生物素化抗体洗净后,加入HRP标记链亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人,小鼠,大鼠 H2B蛋白呈正相关。用酶标仪在450 nm波长下测定吸光度(OD值),通过绘制标准曲线计算出样品浓度。 |

关注微信公众号