货号:YLH530
价格: ¥1800
| 名称 | M-07e 人巨细胞白血病细胞(STR鉴定) |
| 别名 | M-07E;M-O7e;M07-e;M07e;Mo7e;MO7e;M07E;MO7E |
| 种属 | 人 |
| 细胞形态 | 单个圆形细胞和一些小细胞团,少数细胞轻度贴壁 |
| 生长特性 | 半贴半悬 |
| 培养条件 | RPMI-1640+10% FBS+8 ng/mL GM-CSF+1% P/S。气相:空气,95%;二氧化碳,5%。温度:37℃。湿度:70%-80% |
| 推荐传代比例 | 5×10⁵-1×10⁶ cells/mL |
| 换液频率 | 2-3次/周 |
| 冻存条件 | 冻存液:90% FBS+10% DMSO;温度:液氮 |
| 背景描述 | Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available. |
| 组织来源 | 外周血 |
| 生物安全等级 | BSL-1 |

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