货号:YLH547
价格: ¥2000
| 名称 | NCI-H526 人小细胞肺癌细胞(STR鉴定) |
| 别名 | H526;H-526;NCIH526 |
| 种属 | 人 |
| 细胞形态 | 圆形聚团 |
| 生长特性 | 悬浮生长 |
| 培养条件 | RMPI-1640+10% FBS+1% P/S。气相:空气,95%;二氧化碳,5%。温度:37℃。湿度:70%-80% |
| 推荐传代比例 | 1:3-1:6 |
| 换液频率 | 2-3次/周 |
| 冻存条件 | 冻存液:90% FBS+10% DMSO;温度:液氮 |
| 背景描述 | NCI-H526 [H526] is an epithelial cell line that was isolated from the lungs of a 55-year-old, White male with carcinoma.NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase. They do not express L-dopa carboxylase or bombesin-like immunoreactivity. These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc. N-myc is amplified and p75 c-myb expression was observed. NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1. Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected. RB protein was not detected. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. Abnormally sized mRNA was present. |
| 组织来源 | 肺 |
| 生物安全等级 | BSL-1 |

关注微信公众号