货号:YLH574
价格: ¥2000
| 名称 | Mino 人外周血套细胞淋巴瘤细胞(STR鉴定) |
| 种属 | 人 |
| 细胞形态 | 淋巴母细胞样 |
| 生长特性 | 悬浮生长 |
| 培养条件 | RMPI-1640+20% FBS+1% P/S。气相:空气,95%;二氧化碳,5%。温度:37℃。湿度:70%-80% |
| 推荐传代比例 | 1:2-1:3 |
| 换液频率 | 2-3次/周 |
| 冻存条件 | 冻存液:90% FBS+10% DMSO;温度:液氮 |
| 背景描述 | Mino cells are lymphoblasts isolated from the peripheral blood of a 64-year-old, White male with mantle cell lymphoma. It can be used for immunology research. The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL. Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1). |
| 组织来源 | 外周血 |
| 生物安全等级 | BSL-1 |

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